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34 KiB
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{"experiment":{"id":436,"name":"Transformation of E. coli with electroporation","description":"Electroporation is the use of high-voltage electric shocks to introduce DNA into cells. It can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression and, because it requires fewer steps, can be easier than alternate techniques.","project_id":223,"created_by_id":202,"last_modified_by_id":202,"archived":false,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2018-11-13T09:57:55.843Z","updated_at":"2018-12-13T15:44:35.608Z","workflowimg_file_name":"wimg20181213-1-tptre.png","workflowimg_content_type":"image/png","workflowimg_file_size":3029,"workflowimg_updated_at":"2018-12-13T15:44:35.608Z","uuid":"e2831f4b-e14f-4fba-94ac-5c601db534a3"},"my_modules":[{"my_module":{"id":2164,"name":"DNA quantification","due_date":null,"description":null,"x":208,"y":212,"my_module_group_id":null,"created_at":"2018-11-13T12:41:42.309Z","updated_at":"2018-12-13T13:50:26.475Z","archived":true,"archived_on":"2018-12-13T13:50:26.000Z","created_by_id":202,"last_modified_by_id":202,"archived_by_id":202,"restored_by_id":null,"restored_on":null,"nr_of_assigned_samples":0,"workflow_order":-1,"experiment_id":436,"state":"uncompleted","completed_on":null,"electronic_signature_status":"unlocked","electronic_signature_status_locked_at":null},"outputs":[],"my_module_tags":[],"task_comments":[],"my_module_repository_rows":[],"user_my_modules":[],"protocols":[{"protocol":{"id":3506,"name":null,"authors":null,"description":null,"added_by_id":null,"my_module_id":2164,"team_id":1,"protocol_type":"unlinked","parent_id":null,"parent_updated_at":null,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2018-11-13T12:41:42.499Z","updated_at":"2018-11-23T14:05:29.127Z","published_on":null,"nr_of_linked_children":0},"protocol_protocol_keywords":[],"steps":[{"step":{"id":4807,"name":"Run on a mini gel of 0.7% agarose, along with serial dilutions of full-length, linear λDNA and HindIII digests of λDNA.","description":"\u003c!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\"\u003e","position":1,"completed":false,"completed_on":null,"user_id":202,"created_at":"2018-11-23T14:04:42.528Z","updated_at":"2018-11-23T14:05:28.982Z","last_modified_by_id":202,"protocol_id":3506},"checklists":[],"step_comments":[],"step_assets":[],"assets":[],"step_tables":[],"tables":[]},{"step":{"id":4806,"name":"2µl of mini-prepped DNA is digested with NotI","description":"\u003c!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\"\u003e","position":0,"completed":false,"completed_on":null,"user_id":202,"created_at":"2018-11-23T14:04:19.288Z","updated_at":"2018-11-23T14:05:28.996Z","last_modified_by_id":202,"protocol_id":3506},"checklists":[],"step_comments":[],"step_assets":[],"assets":[],"step_tables":[],"tables":[]},{"step":{"id":4809,"name":"After photography, the intensities of the BAC samples are compared with those of the standards.","description":"\u003c!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\"\u003e","position":3,"completed":false,"completed_on":null,"user_id":202,"created_at":"2018-11-23T14:05:09.377Z","updated_at":"2018-11-23T14:05:28.955Z","last_modified_by_id":202,"protocol_id":3506},"checklists":[],"step_comments":[],"step_assets":[],"assets":[],"step_tables":[],"tables":[]},{"step":{"id":4810,"name":"For electroporation, samples are diluted in water and stored at 4°C.","description":"\u003c!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\"\u003e","position":4,"completed":false,"completed_on":null,"user_id":202,"created_at":"2018-11-23T14:05:24.445Z","updated_at":"2018-11-23T14:05:28.940Z","last_modified_by_id":202,"protocol_id":3506},"checklists":[],"step_comments":[],"step_assets":[],"assets":[],"step_
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