{"experiment":{"id":454,"name":"Bottom-up Proteomics","description":"This template will help you prepare protein samples, followed by the cleanup and mass spectrometry analysis. \r\nIn bottom-up proteomics, the protein is first broken up into peptides, either by chemical or enzymatic digestion. In the first step, sample preparation is required after gel electrophoresis, liquid chromatography or affinity capture. Samples can (optionally) be enriched at the peptide level. With mass spectrometry, in the end, you can identify and characterize biological molecules.","project_id":223,"created_by_id":202,"last_modified_by_id":202,"archived":false,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2018-11-26T08:55:55.988Z","updated_at":"2019-01-29T09:46:44.060Z","workflowimg_file_name":"wimg20181219-1-nlf5rk.png","workflowimg_content_type":"image/png","workflowimg_file_size":4681,"workflowimg_updated_at":"2019-01-29T09:46:44.060Z","uuid":"8c69cf68-9330-4c6c-9b8b-86043383a825"},"my_modules":[{"my_module":{"id":2274,"name":"Protein preparation: Affinity Capture","due_date":null,"description":null,"x":0,"y":32,"my_module_group_id":1179,"created_at":"2018-11-27T17:13:44.860Z","updated_at":"2018-12-19T09:02:54.892Z","archived":false,"archived_on":null,"created_by_id":202,"last_modified_by_id":202,"archived_by_id":null,"restored_by_id":null,"restored_on":null,"nr_of_assigned_samples":0,"workflow_order":0,"experiment_id":454,"state":"uncompleted","completed_on":null},"outputs":[{"id":5981,"input_id":2345,"output_id":2274}],"my_module_tags":[],"task_comments":[],"my_module_repository_rows":[],"user_my_modules":[],"protocols":[{"protocol":{"id":3704,"name":null,"authors":null,"description":null,"added_by_id":null,"my_module_id":2274,"team_id":1,"protocol_type":"unlinked","parent_id":null,"parent_updated_at":null,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2018-11-27T17:13:44.867Z","updated_at":"2019-01-31T08:29:28.974Z","published_on":null,"nr_of_linked_children":0},"protocol_protocol_keywords":[],"steps":[{"step":{"id":4872,"name":"Elute protein from the matrix","description":"\u003c!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\"\u003e\n\u003chtml\u003e\u003cbody\u003e\u003cdiv\u003eDown below are the guidelines for elution of proteins.\u003cbr\u003e\u003cbr\u003e\n\u003c/div\u003e\u003c/body\u003e\u003c/html\u003e","position":1,"completed":false,"completed_on":null,"user_id":202,"created_at":"2018-11-27T17:40:37.750Z","updated_at":"2018-12-24T07:26:44.813Z","last_modified_by_id":202,"protocol_id":3704},"checklists":[{"checklist":{"id":858,"name":"Guidelines:","step_id":4872,"created_at":"2018-11-27T17:40:37.753Z","updated_at":"2018-12-20T14:36:53.748Z","created_by_id":null,"last_modified_by_id":null},"checklist_items":[{"id":3564,"text":"Use a 5× volume of 0.1 M glycine, pH 2.5.","checked":false,"checklist_id":858,"created_at":"2018-11-27T17:40:37.755Z","updated_at":"2018-11-27T17:40:37.755Z","created_by_id":null,"last_modified_by_id":null,"position":0},{"id":3565,"text":"Apply the elution buffer and allow the buffer to thoroughly wet the matrix for 5 to 10 min.","checked":false,"checklist_id":858,"created_at":"2018-11-27T17:40:37.764Z","updated_at":"2018-11-27T17:40:37.764Z","created_by_id":null,"last_modified_by_id":null,"position":1},{"id":3566,"text":"Collect the eluate.","checked":false,"checklist_id":858,"created_at":"2018-11-27T17:40:37.772Z","updated_at":"2018-11-27T17:40:37.772Z","created_by_id":null,"last_modified_by_id":null,"position":2},{"id":3567,"text":"To enhance recovery, wash the matrix with an additional 5× volume of 0.1 M glycine, pH 2.5.","checked":false,"checklist_id":858,"created_at":"2018-11-27T17:40:37.780Z","updated_at":"2018-11-27T17:40:37.780Z","created_by_id":null,"last_modified_by_id":null,"position":3},{"id":3568,"text":"Adjust eluate to neutral pH by addition of an equal volume of 200 mM NH4HCO3.","checked":false,"checklist_id":858,"created_
