{"experiment":{"id":497,"name":"SDS-PAGE for Protein Characterization","description":"This experiment template will help you separate proteins based on their molecular weight. \r\nProteins are separated by electrophoresis through a gel matrix. Smaller proteins migrate faster due to less resistance from the gel matrix. Other influences on the rate of migration through the gel matrix include the structure and charge of the proteins. In SDS-PAGE, the use of sodium dodecyl sulfate (SDS, also known as sodium lauryl sulfate) and polyacrylamide gel largely eliminates the influence of the structure and charge, and proteins are separated solely based on polypeptide chain length.\r\nStaining with Coomassie blue dyes is commonly used to stain proteins and visualize them. But if you want to identify specific proteins (using specific antibodies) a better technique to use is a Western blot.","project_id":223,"created_by_id":202,"last_modified_by_id":202,"archived":false,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2018-12-24T10:56:55.416Z","updated_at":"2019-01-28T15:15:24.331Z","workflowimg_file_name":"wimg20181224-1-1hyikrd.png","workflowimg_content_type":"image/png","workflowimg_file_size":2244,"workflowimg_updated_at":"2019-01-28T15:15:24.331Z","uuid":"3363b358-1478-402c-8597-57739d3fb2e3"},"my_modules":[{"my_module":{"id":2388,"name":"Western Blot","due_date":null,"description":null,"x":99,"y":0,"my_module_group_id":1195,"created_at":"2018-12-24T13:08:01.834Z","updated_at":"2018-12-24T13:43:31.275Z","archived":false,"archived_on":null,"created_by_id":202,"last_modified_by_id":202,"archived_by_id":null,"restored_by_id":null,"restored_on":null,"nr_of_assigned_samples":0,"workflow_order":3,"experiment_id":497,"state":"uncompleted","completed_on":null},"outputs":[],"my_module_tags":[],"task_comments":[],"my_module_repository_rows":[],"user_my_modules":[],"protocols":[{"protocol":{"id":3905,"name":null,"authors":null,"description":null,"added_by_id":null,"my_module_id":2388,"team_id":1,"protocol_type":"unlinked","parent_id":null,"parent_updated_at":null,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2018-12-24T13:08:01.843Z","updated_at":"2019-02-20T07:31:00.167Z","published_on":null,"nr_of_linked_children":0},"protocol_protocol_keywords":[],"steps":[{"step":{"id":5264,"name":"Probing with antibodies and detection of the bands","description":"\u003c!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\"\u003e\n\u003chtml\u003e\u003cbody\u003e\n\u003cp\u003e\u003cstrong\u003eBlocking buffer:\u003c/strong\u003e\u003c/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e1-3% BSA in PBS-T:\u003c/strong\u003eBovine serum albumin (BSA) is used as a blocking agent.\u003c/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e1-5% skim milk in PBS-T\u003c/strong\u003eSkim milk is more effective in blocking and used frequently. However, it can interfere with specific antigen-antibody reactions.\u003cem\u003e\u003cstrong\u003eSkim milk contains the \u003c/strong\u003e\u003cstrong\u003ephosphoprotein \u003c/strong\u003e\u003cstrong\u003ecasein and therefore is not suitable for the detection of phosphoproteins.\u003c/strong\u003e\u003c/em\u003e\n\u003c/li\u003e\n\u003c/ul\u003e\n\u003c/body\u003e\u003c/html\u003e","position":2,"completed":false,"completed_on":null,"user_id":202,"created_at":"2018-12-24T13:33:23.306Z","updated_at":"2018-12-24T13:33:53.668Z","last_modified_by_id":202,"protocol_id":3905},"checklists":[{"checklist":{"id":974,"name":"Guidelines","step_id":5264,"created_at":"2018-12-24T13:33:23.308Z","updated_at":"2018-12-24T13:33:23.308Z","created_by_id":null,"last_modified_by_id":null},"checklist_items":[{"id":4013,"text":"Place the membrane in blocking buffer and incubate at room temperature for 1 hour (or at 4°C overnight) with shaking.","checked":false,"checklist_id":974,"created_at":"2018-12-24T13:33:23.310Z","updated_at":"2018-12-24T13:33:23.310Z","created_by_id":null,"last
