mirror of
https://github.com/scinote-eln/scinote-web.git
synced 2024-09-29 19:36:20 +08:00
1 line
72 KiB
JSON
1 line
72 KiB
JSON
|
{"experiment":{"id":430,"name":"Determination of (MIC) by agar and broth dilution","description":"Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation. Dilution methods are used to MICs of antimicrobial agents and are the reference methods for antimicrobial susceptibility testing.","project_id":223,"created_by_id":202,"last_modified_by_id":202,"archived":false,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2018-11-09T08:38:03.789Z","updated_at":"2018-12-19T13:28:42.405Z","workflowimg_file_name":"wimg20181219-1-19cqech.png","workflowimg_content_type":"image/png","workflowimg_file_size":4423,"workflowimg_updated_at":"2018-12-19T13:28:42.405Z","uuid":"f1764340-3adc-4147-ae40-fa82ad16e87a"},"my_modules":[{"my_module":{"id":2128,"name":"Antimicrobial susceptibility testing _agar plates","due_date":null,"description":null,"x":219,"y":80,"my_module_group_id":null,"created_at":"2018-11-09T12:35:59.040Z","updated_at":"2018-12-17T14:30:28.017Z","archived":true,"archived_on":"2018-12-17T14:30:27.000Z","created_by_id":202,"last_modified_by_id":202,"archived_by_id":202,"restored_by_id":null,"restored_on":null,"nr_of_assigned_samples":0,"workflow_order":-1,"experiment_id":430,"state":"uncompleted","completed_on":null,"electronic_signature_status":"unlocked","electronic_signature_status_locked_at":null},"outputs":[],"my_module_tags":[],"task_comments":[],"my_module_repository_rows":[],"user_my_modules":[],"protocols":[{"protocol":{"id":3454,"name":null,"authors":null,"description":null,"added_by_id":null,"my_module_id":2128,"team_id":1,"protocol_type":"unlinked","parent_id":null,"parent_updated_at":null,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2018-11-09T12:35:59.049Z","updated_at":"2018-12-17T14:26:00.134Z","published_on":null,"nr_of_linked_children":0},"protocol_protocol_keywords":[],"steps":[{"step":{"id":4371,"name":"Bacterial suspension","description":"\u003c!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\"\u003e\n\u003chtml\u003e\u003cbody\u003e\u003cp\u003eMix the bacterial suspension, adjusted to 1x10\u003csup\u003e8\u003c/sup\u003e cfu/ml from \u003cspan class=\"atwho-inserted\" contenteditable=\"false\" data-atwho-at-query=\"#gro\"\u003e[#Growth method~tsk~YI]\u003c/span\u003e or \u003cspan class=\"atwho-inserted\" contenteditable=\"false\" data-atwho-at-query=\"#colo\"\u003e[#Colony suspension~tsk~YH]\u003c/span\u003e by vortexing and dilute it 1:10 into a cavity of a sterile 96-well microtiter plate by pipetting 10 ml into a well containing 90 ml of sterile broth or saline.\u003c/p\u003e\u003c/body\u003e\u003c/html\u003e","position":0,"completed":false,"completed_on":null,"user_id":202,"created_at":"2018-11-09T12:51:56.190Z","updated_at":"2018-11-09T12:53:27.876Z","last_modified_by_id":202,"protocol_id":3454},"checklists":[],"step_comments":[],"step_assets":[],"assets":[],"step_tables":[],"tables":[]},{"step":{"id":4372,"name":"Repeat for each bacterial sample","description":"\u003c!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\"\u003e\n\u003chtml\u003e\u003cbody\u003e\u003cp\u003eRepeat for each bacterial isolate to be tested. Be sure to make a note of the content of each well and to inoculate the microtiter plate in a way that the inocula can be transferred to the agar plates using a 48-pin replicator. \u003cbr\u003eFor up to 48 tests, inoculate only within rows A–H, columns 1–6 of the microtiter plate. As an alternative to the replicator, a multichannel micropipette set at 1µl can be used to deliver the spots.\u003cbr\u003eMake sure that the required number of bacterial cells is going to be transferred. A 48-pin replicator\u003cbr\u003ewith 1.5 mm pins delivers 1 µl. The final inoculum for a spot with a size of 5–8 mm should deliver the desired cell density of a
|