{"experiment":{"id":423,"name":"Antibody Purification","description":"Antibody purification involves selective enrichment or specific isolation of antibodies from serum, ascites fluid or cell culture supernatant of a hybridoma cell line. Purification methods range from very crude to highly specific.\r\nOnce they are purified (and possibly after labelling them with an enzyme or fluorescent tag), these antibodies can be used directly to probe the specific antigen in Western blotting, ELISA and other applications.","project_id":223,"created_by_id":202,"last_modified_by_id":202,"archived":false,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2018-11-08T10:28:48.389Z","updated_at":"2018-12-21T13:26:16.207Z","workflowimg_file_name":"wimg20181221-1-12rsihs.png","workflowimg_content_type":"image/png","workflowimg_file_size":1460,"workflowimg_updated_at":"2018-12-21T13:26:16.207Z","uuid":"37198411-2ad0-4198-b680-47f3b455ce0d"},"my_modules":[{"my_module":{"id":2097,"name":"Polishing","due_date":null,"description":null,"x":72,"y":0,"my_module_group_id":1190,"created_at":"2018-11-08T10:28:49.322Z","updated_at":"2018-12-21T13:25:20.391Z","archived":false,"archived_on":null,"created_by_id":202,"last_modified_by_id":null,"archived_by_id":null,"restored_by_id":null,"restored_on":null,"nr_of_assigned_samples":0,"workflow_order":2,"experiment_id":423,"state":"uncompleted","completed_on":null},"outputs":[{"id":6024,"input_id":2098,"output_id":2097}],"my_module_tags":[],"task_comments":[],"my_module_repository_rows":[],"user_my_modules":[],"protocols":[{"protocol":{"id":3407,"name":null,"authors":null,"description":null,"added_by_id":null,"my_module_id":2097,"team_id":1,"protocol_type":"unlinked","parent_id":null,"parent_updated_at":null,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2018-11-08T10:28:49.390Z","updated_at":"2019-02-20T07:39:44.494Z","published_on":null,"nr_of_linked_children":0},"protocol_protocol_keywords":[],"steps":[{"step":{"id":4287,"name":"Before applying a new sample","description":"\u003c!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\"\u003e\n\u003chtml\u003e\u003cbody\u003e\u003cp\u003eRe-equilibrate column with buffer until the baseline of the monitored signal is stable. Proteins can be monitored at\u003cstrong\u003e 280 nm.\u003c/strong\u003e\u003c/p\u003e\u003c/body\u003e\u003c/html\u003e","position":2,"completed":false,"completed_on":null,"user_id":202,"created_at":"2018-11-08T10:28:49.408Z","updated_at":"2018-12-21T13:31:50.804Z","last_modified_by_id":202,"protocol_id":3407},"checklists":[],"step_comments":[],"step_assets":[],"assets":[],"step_tables":[],"tables":[]},{"step":{"id":4290,"name":"Polishing","description":"\u003c!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\"\u003e\n\u003chtml\u003e\u003cbody\u003e\u003cp\u003e\u003cstrong\u003eEquilibrate with 2 column volumes of the buffer\u003c/strong\u003e at a recommended flow rate (See the table). Apply a sample volume equivalent to between \u003cstrong\u003e0.3%\u003c/strong\u003e and \u003cstrong\u003e4%\u003c/strong\u003e of the bed volume. For most applications, the sample volume should not exceed \u003cstrong\u003e2%\u003c/strong\u003e to achieve high resolution. Smaller sample volumes generally lead to an improved resolution.\u003cbr\u003e\u003cstrong\u003eElute with 1 column volume of buffersolution\u003c/strong\u003e (\u003cstrong\u003e10\u003c/strong\u003e to \u003cstrong\u003e50 mM\u003c/strong\u003e sodium phosphate, \u003cstrong\u003e150 mM\u003c/strong\u003e sodium chloride, \u003cstrong\u003epH 7.0\u003c/strong\u003e to \u003cstrong\u003e7.4\u003c/strong\u003e, or select the buffer in which the sample should be stored or solubilized for the next step). The sample should be fully dissolved. Centrifuge or filter to remove particulate matter.\u003c/p\u003e\u003c/body\u003e\u003c/html\u003e","position":1,"completed":false,"complet
