{"experiment":{"id":436,"name":"Transformation of E. coli with electroporation","description":"Electroporation is the use of high-voltage electric shocks to introduce DNA into cells. It can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression and, because it requires fewer steps, can be easier than alternate techniques.","project_id":223,"created_by_id":202,"last_modified_by_id":202,"archived":false,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2018-11-13T09:57:55.843Z","updated_at":"2018-12-13T15:44:35.608Z","workflowimg_file_name":"wimg20181213-1-tptre.png","workflowimg_content_type":"image/png","workflowimg_file_size":3029,"workflowimg_updated_at":"2018-12-13T15:44:35.608Z","uuid":"291969a9-c6fc-4964-99cb-2d3953b5356c"},"my_modules":[{"my_module":{"id":2163,"name":"Preparation of DNA","due_date":null,"description":null,"x":0,"y":16,"my_module_group_id":1165,"created_at":"2018-11-13T11:54:32.038Z","updated_at":"2018-12-13T15:38:36.168Z","archived":false,"archived_on":null,"created_by_id":202,"last_modified_by_id":202,"archived_by_id":null,"restored_by_id":null,"restored_on":null,"nr_of_assigned_samples":0,"workflow_order":0,"experiment_id":436,"state":"uncompleted","completed_on":null,"electronic_signature_status":"unlocked","electronic_signature_status_locked_at":null},"outputs":[{"id":5918,"input_id":2165,"output_id":2163}],"my_module_tags":[],"task_comments":[],"my_module_repository_rows":[],"user_my_modules":[],"protocols":[{"protocol":{"id":3505,"name":null,"authors":null,"description":null,"added_by_id":null,"my_module_id":2163,"team_id":1,"protocol_type":"unlinked","parent_id":null,"parent_updated_at":null,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2018-11-13T11:54:32.112Z","updated_at":"2018-12-24T08:31:00.304Z","published_on":null,"nr_of_linked_children":0},"protocol_protocol_keywords":[],"steps":[{"step":{"id":5186,"name":"Growth of clones","description":"\u003c!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\"\u003e\n\u003chtml\u003e\u003cbody\u003e\u003cp\u003ePlasmid DNA isolation procedure by Birnboim and Doly (1979). The method has been used principally with plasmid pBR322 and its derivatives in E.coli strains HB101, RRI and SK 15921. \u003cbr\u003e\u003cbr\u003e\u003c/p\u003e\u003c/body\u003e\u003c/html\u003e","position":0,"completed":false,"completed_on":null,"user_id":202,"created_at":"2018-12-13T09:33:57.218Z","updated_at":"2018-12-21T09:14:37.945Z","last_modified_by_id":202,"protocol_id":3505},"checklists":[{"checklist":{"id":955,"name":"Guidelines:","step_id":5186,"created_at":"2018-12-21T09:14:37.947Z","updated_at":"2018-12-21T09:14:37.947Z","created_by_id":null,"last_modified_by_id":null},"checklist_items":[{"id":3922,"text":"Selected clones are grown in 2.5 ml of L-broth containing 100 ug/ml ampicillin in 6-ml vials.","checked":false,"checklist_id":955,"created_at":"2018-12-21T09:14:37.950Z","updated_at":"2018-12-21T09:14:37.950Z","created_by_id":null,"last_modified_by_id":null,"position":0},{"id":3923,"text":"After 18 h incubation, 0.5 ml of culture is transferred to a 1.5 ml Eppendorf tube for plasmid extraction.","checked":false,"checklist_id":955,"created_at":"2018-12-21T09:14:37.957Z","updated_at":"2018-12-21T09:14:37.957Z","created_by_id":null,"last_modified_by_id":null,"position":1},{"id":3924,"text":"The remainder is stored at -20°C after the addition of glycerol to 40%.","checked":false,"checklist_id":955,"created_at":"2018-12-21T09:14:37.964Z","updated_at":"2018-12-21T09:14:37.964Z","created_by_id":null,"last_modified_by_id":null,"position":2}]}],"step_comments":[],"step_assets":[],"assets":[],"step_tables":[],"tables":[]},{"step":{"id":4441,"name":"Resuspension and storage","description":"\u003c!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\"\u003e\n\u003chtml\u003e\u003cbody\u003e\n\u003cp\u003eTheplasmi
