{ "experiment": { "id": 430, "name": "Determination of (MIC) by agar and broth dilution", "description": "With this template, minimum inhibitory concentrations (MICs) can be determined. MICs are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation. \r\nThe aim of broth and agar dilution methods is to determine the lowest concentration of the assayed antimicrobial agent (minimal inhibitory concentration, MIC) that, under defined test conditions, inhibits the visible growth of the bacterium being investigated.MIC values are used to determine the susceptibilities of bacteria to drugs and also to evaluate the activity of new antimicrobial agents. Agar dilution involves the incorporation of different concentrations of the antimicrobial substance into a nutrient agar medium followed by the application of a standardized number of cells to the surface of the agar plate. \r\nDilution methods are used to MICs of antimicrobial agents and are the reference methods for antimicrobial susceptibility testing.", "project_id": 223, "created_by_id": 202, "last_modified_by_id": 202, "archived": false, "archived_by_id": null, "archived_on": null, "restored_by_id": null, "restored_on": null, "created_at": "2018-11-09T08:38:03.789Z", "updated_at": "2019-02-07T14:55:25.517Z", "uuid": "f2949887-1f9e-4eb8-b645-ebbdd8808caf" }, "my_modules": [ { "my_module": { "id": 2135, "name": "Troubleshooting of MIC determination", "due_date": null, "description": null, "x": 106, "y": 26, "my_module_group_id": null, "created_at": "2018-11-09T14:43:02.935Z", "updated_at": "2018-11-09T14:43:31.822Z", "archived": false, "archived_on": null, "created_by_id": 202, "last_modified_by_id": 202, "archived_by_id": null, "restored_by_id": null, "restored_on": null, "workflow_order": -1, "experiment_id": 430, "state": "uncompleted", "completed_on": null }, "outputs": [], "my_module_tags": [], "task_comments": [], "my_module_repository_rows": [], "user_my_modules": [], "protocols": [ { "protocol": { "id": 3461, "name": null, "authors": null, "description": null, "added_by_id": null, "my_module_id": 2135, "team_id": 1, "protocol_type": "unlinked", "parent_id": null, "parent_updated_at": null, "archived_by_id": null, "archived_on": null, "restored_by_id": null, "restored_on": null, "created_at": "2018-11-09T14:43:02.943Z", "updated_at": "2019-02-07T13:04:03.800Z", "published_on": null, "nr_of_linked_children": 0 }, "protocol_protocol_keywords": [], "steps": [ { "step": { "id": 4388, "name": "Troubleshooting table", "description": "\n

Look at the table for troubleshooting.

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To confirm that the size of the bacterial inoculum was appropriate, determine the viable count of the bacterial suspension used for preparing the initial inoculum.


", "position": 0, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T13:29:23.180Z", "updated_at": "2018-12-21T12:05:41.321Z", "last_modified_by_id": 202, "protocol_id": 3455 }, "checklists": [ { "checklist": { "id": 967, "name": "Guidelines:", "step_id": 4375, "created_at": "2018-12-21T12:05:41.323Z", "updated_at": "2018-12-21T12:05:41.323Z", "created_by_id": null, "last_modified_by_id": null }, "checklist_items": [ { "id": 3981, "text": "Dilute this suspension 1:100 by pipetting 10 µL into sterile capped Eppendorf tubes containing 990 µL nutrient-rich broth or sterile saline solution (10-2)", "checked": false, "checklist_id": 967, "created_at": "2018-12-21T12:05:41.325Z", "updated_at": "2018-12-21T12:05:41.325Z", "created_by_id": null, "last_modified_by_id": null, "position": 0 }, { "id": 3982, "text": "Dilute this solution sequentially 1:10 three times until you reach a dilution of 10–5.", "checked": false, "checklist_id": 967, "created_at": "2018-12-21T12:05:41.338Z", "updated_at": "2018-12-21T12:05:41.338Z", "created_by_id": null, "last_modified_by_id": null, "position": 1 }, { "id": 3983, "text": "Plate 100 mL of the last two 1:10 dilutions (10–4 to 10–5) evenly onto antibiotic-free nutrient-rich agar plates using a sterile cell spreader.", "checked": false, "checklist_id": 967, "created_at": "2018-12-21T12:05:41.345Z", "updated_at": "2018-12-21T12:05:41.345Z", "created_by_id": null, "last_modified_by_id": null, "position": 2 } ] } ], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] }, { "step": { "id": 4377, "name": "Colonies count", "description": "\n

Count colonies on plates (taking into account only plates with up to 500 colonies).
It is best to determine the relationship between OD600 and the microbial number basing the calculation on plates displaying a number of colonies between 100 and 400. Higher numbers do not accurately represent the original suspension as errors created by coincidence of colonies and nutrient limitation lead to numbers that are lower than expected. Relying on fewer than 100 colonies might, on the other hand, lead to incorrect conclusions due to the addition of statistical and methodological errors while performing the dilutions.

", "position": 2, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T13:44:18.697Z", "updated_at": "2019-02-07T10:32:30.551Z", "last_modified_by_id": 202, "protocol_id": 3455 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] }, { "step": { "id": 4376, "name": "Incubation", "description": "\n\n

Incubation parameters:

\n\n", "position": 1, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T13:41:55.993Z", "updated_at": "2019-02-19T14:40:36.350Z", "last_modified_by_id": 202, "protocol_id": 3455 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] }, { "step": { "id": 4378, "name": "Calculation of CFU", "description": "\n

Calculate the CFU per mL that were in the overnight culture according to the following formula, where N=(Cx10)/10-D (CFU/mL); C= number of colonies per plate; D= number of 1:10 dilution.

Average the results from three tests performed with the microbial isolate and correlate this number with the OD600 value obtained from the overnight culture. This relationship holds true for subsequent cultures of the same bacterium grown in the same way.

", "position": 3, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T13:46:56.839Z", "updated_at": "2019-02-07T10:33:29.603Z", "last_modified_by_id": 202, "protocol_id": 3455 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] } ] } ], "results": [] }, { "my_module": { "id": 2134, "name": "MIC agar dilutions", "due_date": null, "description": null, "x": 70, "y": 26, "my_module_group_id": 1185, "created_at": "2018-11-09T14:35:06.102Z", "updated_at": "2018-12-19T13:28:00.795Z", "archived": false, "archived_on": null, "created_by_id": 202, "last_modified_by_id": 202, "archived_by_id": null, "restored_by_id": null, "restored_on": null, "workflow_order": 5, "experiment_id": 430, "state": "uncompleted", "completed_on": null }, "outputs": [], "my_module_tags": [], "task_comments": [], "my_module_repository_rows": [], "user_my_modules": [], "protocols": [ { "protocol": { "id": 3460, "name": null, "authors": null, "description": null, "added_by_id": null, "my_module_id": 2134, "team_id": 1, "protocol_type": "unlinked", "parent_id": null, "parent_updated_at": null, "archived_by_id": null, "archived_on": null, "restored_by_id": null, "restored_on": null, "created_at": "2018-11-09T14:35:06.105Z", "updated_at": "2019-02-20T07:38:52.274Z", "published_on": null, "nr_of_linked_children": 0 }, "protocol_protocol_keywords": [], "steps": [ { "step": { "id": 4387, "name": "Interpretation", "description": "\n

In order for the test to be valid, the agar plates for the cell count have to be checked to verify that the right number of CFU were used. The presence of around 100–200 colonies on the lower of the two dilutions (10-5) of the initial bacterial suspension is expected when using the correct bacterial suspension density of 1–2x108 CFU/mL. If the cell numbers are within the desired range, the test can be analyzed to determine the MIC. Also, check the antibiotic-free growth control plate. Visible growth needs to occur for the test to be valid.

The MIC is defined as the lowest concentration of the antimicrobial substance that inhibits visible growth of the tested isolate. The growth of a single colony or faint film caused by the inoculum should be disregarded. When growth of the tested organism occurs on all agar plates with antimicrobial agent, the MIC is recorded as greater than the highest concentration tested. The MIC is recorded as less than or equal to the lowest concentration when no growth occurs on any of the agar plates but the growth control.

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Bacterial suspension preparation: Mix the bacterial suspension, adjusted to 1x108 CFU/mL from [#Growth method~tsk~YI]  or  [#Colony suspension~tsk~YH]  by vortexing and dilute it 1:10 into a cavity of a sterile 96-well microtiter plate by pipetting 10 mL into a well containing 90 mL of sterile broth or saline. Repeat for each bacterial isolate to be tested. Be sure to make a note of the content of each well and to inoculate the microtiter plate in a way that the inocula can be transferred to the agar plates using a 48-pin replicator. For up to 48 tests, inoculate only within rows A–H, columns 1–6 of the microtiter plate. As an alternative to the replicator, a multichannel micropipette set at 1µL can be used to deliver the spots. Make sure that the required number of bacterial cells is going to be transferred. A 48-pin replicator with 1.5 mm pins delivers 1 µL. The final inoculum for a spot with a size of 5–8 mm should deliver the desired cell density of around 104 CFU per spot.

Inoculation: Sterilize the 48-pin replicator by soaking the pins in 95% ethanol and passing them through a Bunsen burner flame. Hold the pins in an upright position until the flame extinguishes. Let the pins cool in an inverted position to maintain sterility. Place the sterilized replicator into the microtiter plate to soak the pins and transfer it onto the agar plate. Start by inoculating a growth control plate without antibiotic. Make sure that each agar plate has the same orientation while inoculating and to make a note of the orientation so that spots on the agar plate can be assigned to the respective isolate tested. Inoculate the antibiotic-containing agar plates starting with the lowest concentration. Let the inoculum spots dry at room temperature before inverting the plates. Plate 100 µL of the last two 1:10 dilutions (10–4 to 10–5) evenly onto antibiotic-free nutrient-rich agar plates using a sterile cell spreader.

\nIncubation:
\n\nCount colonies the next day ( [#Cell count~tsk~YL] )", "position": 1, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-12-17T14:27:56.093Z", "updated_at": "2019-02-20T07:39:27.591Z", "last_modified_by_id": 202, "protocol_id": 3458 }, "checklists": [], "step_comments": [], "step_assets": [ { "id": 3089, "step_id": 5200, "asset_id": 3751 } ], "assets": [ { "asset": { "id": 3751, "created_at": "2019-02-20T07:38:33.374Z", "updated_at": "2019-02-20T07:39:27.582Z", "created_by_id": 202, "last_modified_by_id": null, "estimated_size": 12769, "lock": null, "lock_ttl": null, "version": 1, "file_processing": false, "team_id": 1 }, "asset_blob": { "filename": "Replicator.jpg" } } ], "step_tables": [], "tables": [] }, { "step": { "id": 5198, "name": "Broth macrodilutions", "description": "\n\n

Broth dilutions preparation: Add 1 mL of each antibiotic dilution into one test tube for each isolate to be tested. Fill the control tubes with 1 mL sterile broth without an antimicrobial agent. Mix the bacterial suspension well. The suspension should be adjusted to 1x108 CFU mL/L from [#Growth method~tsk~YI]  and [#Colony suspension~tsk~YH]  by vortexing, and dilute it by a factor of 1:100 by adding 200 µL bacterial suspension to 19.8 mL sterile MHB in a sterile 50 mL Erlenmeyer flask to prepare a 20 mL inoculum. Adjust volumes if necessary. 
Inoculation: Inoculate each test tube containing the antibiotic solution and one control test tube (growth control) with 1 mL of the bacterial suspension. This results in the final desired inoculum of 5x105 CFU mL/L.

Incubation:

\n\nCount colonies the next day( [#Cell count~tsk~YL] ).", "position": 0, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-12-17T14:06:59.583Z", "updated_at": "2019-02-19T14:45:31.219Z", "last_modified_by_id": 202, "protocol_id": 3458 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] } ] } ], "results": [] }, { "my_module": { "id": 2131, "name": "Macrodilutions of antibiotics", "due_date": null, "description": null, "x": 0, "y": 35, "my_module_group_id": 1185, "created_at": "2018-11-09T14:09:52.226Z", "updated_at": "2018-12-19T13:28:00.800Z", "archived": false, "archived_on": null, "created_by_id": 202, "last_modified_by_id": 202, "archived_by_id": null, "restored_by_id": null, "restored_on": null, "workflow_order": 0, "experiment_id": 430, "state": "uncompleted", "completed_on": null }, "outputs": [ { "id": 6013, "input_id": 2132, "output_id": 2131 } ], "my_module_tags": [], "task_comments": [], "my_module_repository_rows": [], "user_my_modules": [], "protocols": [ { "protocol": { "id": 3457, "name": null, "authors": null, "description": null, "added_by_id": null, "my_module_id": 2131, "team_id": 1, "protocol_type": "unlinked", "parent_id": null, "parent_updated_at": null, "archived_by_id": null, "archived_on": null, "restored_by_id": null, "restored_on": null, "created_at": "2018-11-09T14:09:52.235Z", "updated_at": "2019-02-19T14:41:27.828Z", "published_on": null, "nr_of_linked_children": 0 }, "protocol_protocol_keywords": [], "steps": [ { "step": { "id": 4382, "name": "Prepare antibiotic dilutions", "description": "\n

Prepare antibiotic dilutions following steps below.

", "position": 0, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T14:16:11.568Z", "updated_at": "2018-12-24T09:07:19.921Z", "last_modified_by_id": 202, "protocol_id": 3457 }, "checklists": [ { "checklist": { "id": 965, "name": "Guideline:", "step_id": 4382, "created_at": "2018-12-21T11:38:45.338Z", "updated_at": "2018-12-21T11:38:45.338Z", "created_by_id": null, "last_modified_by_id": null }, "checklist_items": [ { "id": 3970, "text": "Prepare antibiotic dilutions in sterile MHB in sterile test tubes according to table.", "checked": false, "checklist_id": 965, "created_at": "2018-12-21T11:38:45.340Z", "updated_at": "2018-12-21T11:38:45.340Z", "created_by_id": null, "last_modified_by_id": null, "position": 0 }, { "id": 3971, "text": "As the antibiotic solution is later inoculated with an equal amount of bacteria in broth, the dilutions are prepared at a concentration twice the desired final concentration.", "checked": false, "checklist_id": 965, "created_at": "2018-12-21T11:38:45.347Z", "updated_at": "2018-12-21T11:38:45.347Z", "created_by_id": null, "last_modified_by_id": null, "position": 1 }, { "id": 3972, "text": "Start by dispensing sterile broth into twelve sterile 13x100 mm tubes closed with metal caps. A single 10 mL pipette can be used to pipette the 9 mL and 3 mL volumes of broth into the respective tubes. Use a single 1 mL pipette for pipetting the 1 mL of broth in stages 2, 5, 8 and 11.", "checked": false, "checklist_id": 965, "created_at": "2018-12-21T11:38:45.354Z", "updated_at": "2018-12-21T11:38:45.354Z", "created_by_id": null, "last_modified_by_id": null, "position": 2 }, { "id": 3973, "text": "It is possible to use the same pipette for pipetting 1 mL of the antibiotic stock solution into the first test tube. Mix thoroughly using a vortex mixer.", "checked": false, "checklist_id": 965, "created_at": "2018-12-21T11:38:45.361Z", "updated_at": "2018-12-21T11:38:45.361Z", "created_by_id": null, "last_modified_by_id": null, "position": 3 }, { "id": 3974, "text": "Use separate pipettes/pipette tips when preparing each of the other antibiotic solutions. Mix thoroughly using a vortex mixer.", "checked": false, "checklist_id": 965, "created_at": "2018-12-21T11:38:45.371Z", "updated_at": "2018-12-21T11:38:45.371Z", "created_by_id": null, "last_modified_by_id": null, "position": 4 } ] } ], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [ { "id": 546, "step_id": 4382, "table_id": 683 } ], "tables": [ { "id": 683, "created_at": "2018-12-21T11:38:45.382Z", "updated_at": "2018-12-21T11:48:45.258Z", "created_by_id": 202, "last_modified_by_id": 202, "name": "", "team_id": 1, "contents": "eyJkYXRhIjpbWyJTdGFnZSIsIkFudGltaWNyb2JpYWwgY29uY2VudHJhdGlv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\nLCJTdGFnZSAxMCIsIjEiLCIzIiwiMC4wNiIsIjAuMDMiXV19\n", "data_vector": "JzAuMDMnOjExOSAnMC4wNic6MTExLDExOCAnMC4xMjUnOjEwMywxMTAgJzAu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\nMjMgJ3ZvbHVtZSc6NiwxMg==\n" } ] }, { "step": { "id": 4383, "name": "Labeling of test tubes", "description": "\n

For every bacterial isolate, label twelve sterile 13x100 mm test tubes closed with cotton buds or metal caps with the respective antibiotic concentration to be tested.
Label control tubes for bacterial growth and a single tube for sterility control for the entire measurement.

", "position": 1, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T14:17:30.554Z", "updated_at": "2019-02-19T14:41:27.778Z", "last_modified_by_id": 202, "protocol_id": 3457 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] } ] } ], "results": [] }, { "my_module": { "id": 2127, "name": "Agar plate with antibiotics", "due_date": null, "description": null, "x": 0, "y": 50, "my_module_group_id": 1185, "created_at": "2018-11-09T10:47:34.880Z", "updated_at": "2018-12-19T13:28:00.803Z", "archived": false, "archived_on": null, "created_by_id": 202, "last_modified_by_id": 202, "archived_by_id": null, "restored_by_id": null, "restored_on": null, "workflow_order": 1, "experiment_id": 430, "state": "uncompleted", "completed_on": null }, "outputs": [ { "id": 6012, "input_id": 2132, "output_id": 2127 } ], "my_module_tags": [], "task_comments": [], "my_module_repository_rows": [], "user_my_modules": [], "protocols": [ { "protocol": { "id": 3453, "name": null, "authors": null, "description": null, "added_by_id": null, "my_module_id": 2127, "team_id": 1, "protocol_type": "unlinked", "parent_id": null, "parent_updated_at": null, "archived_by_id": null, "archived_on": null, "restored_by_id": null, "restored_on": null, "created_at": "2018-11-09T10:47:34.945Z", "updated_at": "2019-02-19T14:43:41.236Z", "published_on": null, "nr_of_linked_children": 0 }, "protocol_protocol_keywords": [], "steps": [ { "step": { "id": 4369, "name": "Dilute antibiotic stock", "description": "\n

Dilute the 10 mg/mL antibiotic stock solution 1:10 in sterile broth or water to achieve a 1 mg/mL solution.
Dilute the 1 mg/mL solution 1:10 in sterile broth or water to achieve a 0.1 mg/mL solution

", "position": 2, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T12:10:09.672Z", "updated_at": "2019-02-19T14:43:41.186Z", "last_modified_by_id": 202, "protocol_id": 3453 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] }, { "step": { "id": 4370, "name": "Prepare agar plates", "description": "\n

Prepare agar plates following guidelines below.

", "position": 3, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T12:22:03.852Z", "updated_at": "2018-12-24T09:08:20.489Z", "last_modified_by_id": 202, "protocol_id": 3453 }, "checklists": [ { "checklist": { "id": 966, "name": "Guidelines:", "step_id": 4370, "created_at": "2018-12-21T11:52:37.302Z", "updated_at": "2018-12-21T11:52:37.302Z", "created_by_id": null, "last_modified_by_id": null }, "checklist_items": [ { "id": 3975, "text": "Dispense appropriate amounts of antibiotic solution into the respective containers. Follow table steps.", "checked": false, "checklist_id": 966, "created_at": "2018-12-21T11:52:37.304Z", "updated_at": "2018-12-21T11:52:37.304Z", "created_by_id": null, "last_modified_by_id": null, "position": 0 }, { "id": 3976, "text": "For each agar plate, add 25 mL agar (now at a temperature of 50°C) into the container, mix well (avoid bubbles) and pour 25 ml into a petri dish labelled with the respective antibiotic concentration.", "checked": false, "checklist_id": 966, "created_at": "2018-12-21T11:52:37.311Z", "updated_at": "2018-12-21T11:52:37.311Z", "created_by_id": null, "last_modified_by_id": null, "position": 1 }, { "id": 3977, "text": "Pour a control agar plate without any antibiotic. Adjust the number if necessary.", "checked": false, "checklist_id": 966, "created_at": "2018-12-21T11:52:37.317Z", "updated_at": "2018-12-21T11:52:37.317Z", "created_by_id": null, "last_modified_by_id": null, "position": 2 }, { "id": 3978, "text": "Allow agar to set.", "checked": false, "checklist_id": 966, "created_at": "2018-12-21T11:52:37.324Z", "updated_at": "2018-12-21T11:52:37.324Z", "created_by_id": null, "last_modified_by_id": null, "position": 3 }, { "id": 3979, "text": "Dry the surface of the agar plates either in an incubator or in a laminar airflow hood for 30 min. Leave the lid ajar.", "checked": false, "checklist_id": 966, "created_at": "2018-12-21T11:52:37.330Z", "updated_at": "2018-12-21T11:52:37.330Z", "created_by_id": null, "last_modified_by_id": null, "position": 4 }, { "id": 3980, "text": "Mark the bottom of the agar plates to define an orientation.", "checked": false, "checklist_id": 966, "created_at": "2018-12-21T11:52:37.336Z", "updated_at": "2018-12-21T11:52:37.336Z", "created_by_id": null, "last_modified_by_id": null, "position": 5 } ] } ], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [ { "id": 545, "step_id": 4370, "table_id": 682 } ], "tables": [ { "id": 682, "created_at": "2018-12-20T13:07:47.021Z", "updated_at": "2018-12-24T09:08:20.452Z", "created_by_id": 202, "last_modified_by_id": 202, "name": "", "team_id": 1, "contents": "eyJkYXRhIjpbWyJBbnRpbWljcm9iaWFsIGNvbmNlbnRyYXRpb24gKG1nL0wp\nIiwiVm9sdW1lIG9mIGFudGliaW90aWMgc3RvY2sgc29sdXRpb24gKM68TCki\nLCJGaW5hbCBjb25jZW50cmF0aW9uIHdoZW4gYWRkaW5nIDI1bUwgb2YgYWdh\nciJdLFsiMTAiLCIzMjAiLCIxMjgiXSxbIjEwIiwiMTYwIiwiNjQiXSxbIjEw\nIiwiODAiLCIzMiJdLFsiMTAiLCI0MCIsIjE2Il0sWyIxIiwiMjAwIiwiOCJd\nLFsiMSIsIjEwMCIsIjQiXSxbIjEiLCI1MCIsIjIiXSxbIjAuMSIsIjI1MCIs\nIjEiXSxbIjAuMSIsIjEyNSIsIjAuNSJdLFsiMC4xIiwiNjIuNSIsIjAuMjUi\nXSxbIjAuMSIsIjMxLjI1IiwiMS4xMjUiXV19\n", "data_vector": "JzAuMSc6MzksNDIsNDUsNDggJzAuMjUnOjQ3ICcwLjUnOjQ0ICcxJzozMCwz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\nZW4nOjEz\n" } ] }, { "step": { "id": 5197, "name": "Preparation and autoclaving of media", "description": "\n\n
\n
\n
\n

Prepare MHA medium following manufacturer’s instructions. Autoclaving at 121.1°C for 15 minutes at 1 bar. Cool medium to 50°C after that. Around 25 mL is necessary to pour one 15 100 mm petri dish to produce the required depth of 3–4 mm


\n
\n
\n
\n
 
\n", "position": 0, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-12-17T14:00:24.509Z", "updated_at": "2019-02-19T14:42:34.757Z", "last_modified_by_id": 202, "protocol_id": 3453 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] }, { "step": { "id": 4367, "name": "Calculate the amount of antibiotic solutions", "description": "\n

As the medium cools down, calculate the amount of antibiotic solutions (10, 1 and 0.1 mg/L) needed. The table is an example of the volumes needed for a concentration range between 0.125 and 128 mg/L for one 25 mL agar plate per concentration. Adjust if a different concentration range is needed. The volumes of antibiotic and agar can also be varied depending on the number of plates to be poured. As each agar plate can be used for up to 48 tests, more than one plate might be necessary if a larger amount of isolates is going to be tested.

Label sterile containers appropriately (glass Erlenmeyer flasks closed with metal caps, tinfoil caps or cotton buds) with the final antibiotic concentration.

", "position": 1, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T11:07:28.842Z", "updated_at": "2019-02-19T14:43:10.090Z", "last_modified_by_id": 202, "protocol_id": 3453 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [ { "id": 544, "step_id": 4367, "table_id": 681 } ], "tables": [ { "id": 681, "created_at": "2018-12-20T13:03:47.611Z", "updated_at": "2019-02-19T14:43:10.075Z", "created_by_id": 202, "last_modified_by_id": 202, "name": "", "team_id": 1, "contents": "eyJkYXRhIjpbWyJBbnRpbWljcm9iaWFsIGNvbmNlbnRyYXRpb24gKG1nL0wp\nIiwiVm9sdW1lIG9mIGFudGliaW90aWMgc3RvY2sgc29sdXRpb24gKM68TCki\nLCJGaW5hbCBjb25jZW50cmF0aW9uIHdoZW4gYWRkaW5nIDI1bUwgb2YgYWdh\nciJdLFsiMTAiLCIzMjAiLCIxMjgiXSxbIjEwIiwiMTYwIiwiNjQiXSxbIjEw\nIiwiODAiLCIzMiJdLFsiMTAiLCI0MCIsIjE2Il0sWyIxIiwiMjAwIiwiOCJd\nLFsiMSIsIjEwMCIsIjQiXSxbIjEiLCI1MCIsIjIiXSxbIjAuMSIsIjI1MCIs\nIjEiXSxbIjAuMSIsIjEyNSIsIjAuNSJdLFsiMC4xIiwiNjIuNSIsIjAuMjUi\nXSxbIjAuMSIsIjMxLjI1IiwiMS4xMjUiXV19\n", "data_vector": "JzAuMSc6MzksNDIsNDUsNDggJzAuMjUnOjQ3ICcwLjUnOjQ0ICcxJzozMCwz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\nZW4nOjEz\n" } ] } ] } ], "results": [] }, { "my_module": { "id": 2125, "name": "Colony suspension", "due_date": null, "description": null, "x": 0, "y": 0, "my_module_group_id": 1185, "created_at": "2018-11-09T09:15:09.287Z", "updated_at": "2018-12-19T13:28:00.805Z", "archived": false, "archived_on": null, "created_by_id": 202, "last_modified_by_id": 202, "archived_by_id": null, "restored_by_id": null, "restored_on": null, "workflow_order": 3, "experiment_id": 430, "state": "uncompleted", "completed_on": null }, "outputs": [ { "id": 6009, "input_id": 2132, "output_id": 2125 } ], "my_module_tags": [], "task_comments": [], "my_module_repository_rows": [], "user_my_modules": [], "protocols": [ { "protocol": { "id": 3450, "name": null, "authors": null, "description": null, "added_by_id": null, "my_module_id": 2125, "team_id": 1, "protocol_type": "unlinked", "parent_id": null, "parent_updated_at": null, "archived_by_id": null, "archived_on": null, "restored_by_id": null, "restored_on": null, "created_at": "2018-11-09T09:15:09.296Z", "updated_at": "2019-02-20T07:38:05.434Z", "published_on": null, "nr_of_linked_children": 0 }, "protocol_protocol_keywords": [], "steps": [ { "step": { "id": 5202, "name": "Take samples for cell count", "description": "\n

Follow the protocol in [#Cell count~tsk~YL] 

", "position": 4, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-12-17T14:34:48.930Z", "updated_at": "2018-12-17T14:34:48.930Z", "last_modified_by_id": 202, "protocol_id": 3450 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] }, { "step": { "id": 4357, "name": "Isolate preparation", "description": "\n

Follow the guidelines below to prepare an isolate.

", "position": 1, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T09:26:34.638Z", "updated_at": "2018-12-24T08:59:10.687Z", "last_modified_by_id": 202, "protocol_id": 3450 }, "checklists": [ { "checklist": { "id": 962, "name": "Guidelines", "step_id": 4357, "created_at": "2018-12-21T11:09:29.266Z", "updated_at": "2018-12-21T11:09:29.266Z", "created_by_id": null, "last_modified_by_id": null }, "checklist_items": [ { "id": 3957, "text": "For each isolate, select three to five morphologically similar colonies from the fresh agar plate from task", "checked": false, "checklist_id": 962, "created_at": "2018-12-21T11:09:29.268Z", "updated_at": "2018-12-21T11:09:29.268Z", "created_by_id": null, "last_modified_by_id": null, "position": 0 }, { "id": 3958, "text": "Plate preparation", "checked": false, "checklist_id": 962, "created_at": "2018-12-21T11:09:29.276Z", "updated_at": "2018-12-21T11:09:29.276Z", "created_by_id": null, "last_modified_by_id": null, "position": 1 }, { "id": 3959, "text": "Touch the top of each selected colony using a sterile loop or cotton swab.", "checked": false, "checklist_id": 962, "created_at": "2018-12-21T11:09:29.287Z", "updated_at": "2018-12-21T11:09:29.287Z", "created_by_id": null, "last_modified_by_id": null, "position": 2 }, { "id": 3960, "text": "Transfer the growth into a sterile capped glass tube containing sterile broth or saline solution.", "checked": false, "checklist_id": 962, "created_at": "2018-12-21T11:09:29.300Z", "updated_at": "2019-02-07T09:10:36.760Z", "created_by_id": null, "last_modified_by_id": null, "position": 3 }, { "id": 3961, "text": "Mix using a vortex mixer.", "checked": false, "checklist_id": 962, "created_at": "2018-12-21T11:09:29.307Z", "updated_at": "2018-12-21T11:09:29.307Z", "created_by_id": null, "last_modified_by_id": null, "position": 4 } ] } ], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] }, { "step": { "id": 5195, "name": "Pure cultures of bacterial isolates", "description": "\n\n

Growth conditions:

\n\n", "position": 0, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-12-17T13:07:04.214Z", "updated_at": "2019-02-20T07:38:27.031Z", "last_modified_by_id": 202, "protocol_id": 3450 }, "checklists": [ { "checklist": { "id": 961, "name": "Guidelines:", "step_id": 5195, "created_at": "2018-12-21T10:50:36.576Z", "updated_at": "2018-12-21T10:50:36.576Z", "created_by_id": null, "last_modified_by_id": null }, "checklist_items": [ { "id": 3954, "text": "Prepare media (store at 4°C)", "checked": false, "checklist_id": 961, "created_at": "2018-12-21T10:50:36.577Z", "updated_at": "2018-12-21T10:50:36.577Z", "created_by_id": null, "last_modified_by_id": null, "position": 0 }, { "id": 3955, "text": "Prepare antibiotic stock solutions", "checked": false, "checklist_id": 961, "created_at": "2018-12-21T10:50:36.584Z", "updated_at": "2018-12-21T10:50:36.584Z", "created_by_id": null, "last_modified_by_id": null, "position": 1 }, { "id": 3956, "text": "Streak the bacterial isolates to be tested onto nutrient-rich agar plates without inhibitor to obtain single colonies", "checked": false, "checklist_id": 961, "created_at": "2018-12-21T10:50:36.592Z", "updated_at": "2018-12-21T10:50:36.592Z", "created_by_id": null, "last_modified_by_id": null, "position": 2 } ] } ], "step_comments": [], "step_assets": [ { "id": 3088, "step_id": 5195, "asset_id": 3750 } ], "assets": [ { "asset": { "id": 3750, "created_at": "2019-02-20T07:38:05.206Z", "updated_at": "2019-02-20T07:38:27.023Z", "created_by_id": 202, "last_modified_by_id": null, "estimated_size": 48768, "lock": null, "lock_ttl": null, "version": 1, "file_processing": false, "team_id": 1 }, "asset_blob": { "filename": "Plate_Streaking.jpg" } } ], "step_tables": [], "tables": [] }, { "step": { "id": 4358, "name": "Assessment of turbidity", "description": "\n

Turbidity can be assessed visually by comparing the test and the McFarland Standard.

Mix the McFarland 0.5 BaSO4 standard vigorously using a vortex mixer. The turbidity is verified measuring the absorbance of the suspension spectrophotometrically. The absorbance should be in the same range as that of the McFarland standard 0.5 (OD625 nm should be at 0.08–0.13).

", "position": 2, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T10:11:24.463Z", "updated_at": "2019-02-07T10:31:21.634Z", "last_modified_by_id": 202, "protocol_id": 3450 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] }, { "step": { "id": 4359, "name": "Adjustment of suspension turbidity", "description": "\n

Adjust the suspension’s turbidity to that of a McFarland Standard 0.5 by adding sterile distilled water, saline or broth, if the turbidity is too high, or by adding more bacterial material if is too low.

Go back to step 3.

", "position": 3, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T10:13:32.396Z", "updated_at": "2019-02-07T10:31:41.571Z", "last_modified_by_id": 202, "protocol_id": 3450 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] } ] } ], "results": [] }, { "my_module": { "id": 2126, "name": "Growth method", "due_date": null, "description": null, "x": 0, "y": 17, "my_module_group_id": 1185, "created_at": "2018-11-09T10:25:56.551Z", "updated_at": "2018-12-19T13:28:00.808Z", "archived": false, "archived_on": null, "created_by_id": 202, "last_modified_by_id": 202, "archived_by_id": null, "restored_by_id": null, "restored_on": null, "workflow_order": 2, "experiment_id": 430, "state": "uncompleted", "completed_on": null }, "outputs": [ { "id": 6011, "input_id": 2132, "output_id": 2126 } ], "my_module_tags": [], "task_comments": [], "my_module_repository_rows": [], "user_my_modules": [], "protocols": [ { "protocol": { "id": 3452, "name": null, "authors": null, "description": null, "added_by_id": null, "my_module_id": 2126, "team_id": 1, "protocol_type": "unlinked", "parent_id": null, "parent_updated_at": null, "archived_by_id": null, "archived_on": null, "restored_by_id": null, "restored_on": null, "created_at": "2018-11-09T10:25:56.621Z", "updated_at": "2019-02-20T07:37:48.403Z", "published_on": null, "nr_of_linked_children": 0 }, "protocol_protocol_keywords": [], "steps": [ { "step": { "id": 5201, "name": "Take samples for cell count", "description": "\n

Take samples for cell count and follow protocol in [#Cell count~tsk~YL].

", "position": 5, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-12-17T14:33:52.646Z", "updated_at": "2018-12-17T14:33:52.646Z", "last_modified_by_id": 202, "protocol_id": 3452 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] }, { "step": { "id": 4360, "name": "Adjustment of suspension turbidity", "description": "\n

Adjust the suspension’s turbidity to that of a McFarland Standard 0.5 by adding sterile distilled water, saline or broth, if the turbidity is too high, or by adding more bacterial material if is too low.

Go back to step 4.

", "position": 4, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T10:25:56.635Z", "updated_at": "2019-02-19T14:44:57.268Z", "last_modified_by_id": 202, "protocol_id": 3452 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] }, { "step": { "id": 4362, "name": "Isolate preparation", "description": "\n

Follow steps below for isolate preparation.

", "position": 1, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T10:25:56.680Z", "updated_at": "2018-12-24T09:01:33.668Z", "last_modified_by_id": 202, "protocol_id": 3452 }, "checklists": [ { "checklist": { "id": 964, "name": "Guidelines:", "step_id": 4362, "created_at": "2018-12-21T11:14:19.776Z", "updated_at": "2018-12-21T11:14:19.776Z", "created_by_id": null, "last_modified_by_id": null }, "checklist_items": [ { "id": 3965, "text": "For each isolate, select three to five morphologically similar colonies from the fresh agar plate from task .", "checked": false, "checklist_id": 964, "created_at": "2018-12-21T11:14:19.778Z", "updated_at": "2018-12-21T11:14:19.778Z", "created_by_id": null, "last_modified_by_id": null, "position": 0 }, { "id": 3966, "text": "Plate preparation.", "checked": false, "checklist_id": 964, "created_at": "2018-12-21T11:14:19.785Z", "updated_at": "2018-12-21T11:14:19.785Z", "created_by_id": null, "last_modified_by_id": null, "position": 1 }, { "id": 3967, "text": "Touch the top of each selected colony using a sterile loop or cotton swab.", "checked": false, "checklist_id": 964, "created_at": "2018-12-21T11:14:19.791Z", "updated_at": "2018-12-21T11:14:19.791Z", "created_by_id": null, "last_modified_by_id": null, "position": 2 }, { "id": 3968, "text": "Transfer into a tube containing 3–4 mL of a suitable nutrient-rich medium.", "checked": false, "checklist_id": 964, "created_at": "2018-12-21T11:14:19.798Z", "updated_at": "2018-12-21T11:14:19.798Z", "created_by_id": null, "last_modified_by_id": null, "position": 3 }, { "id": 3969, "text": "Mix using a vortex mixer.", "checked": false, "checklist_id": 964, "created_at": "2018-12-21T11:14:19.805Z", "updated_at": "2018-12-21T11:14:19.805Z", "created_by_id": null, "last_modified_by_id": null, "position": 4 } ] } ], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] }, { "step": { "id": 4363, "name": "Pure cultures of bacterial isolates", "description": "\n

Following guidelines below will get you to obtain bacterial isolates.

", "position": 0, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T10:25:56.700Z", "updated_at": "2019-02-20T07:38:26.679Z", "last_modified_by_id": 202, "protocol_id": 3452 }, "checklists": [ { "checklist": { "id": 963, "name": "Guidelines:", "step_id": 4363, "created_at": "2018-12-21T11:12:20.820Z", "updated_at": "2018-12-21T11:12:20.820Z", "created_by_id": null, "last_modified_by_id": null }, "checklist_items": [ { "id": 3962, "text": "Prepare media (store at 4°C).", "checked": false, "checklist_id": 963, "created_at": "2018-12-21T11:12:20.822Z", "updated_at": "2018-12-21T11:12:20.822Z", "created_by_id": null, "last_modified_by_id": null, "position": 0 }, { "id": 3963, "text": "Prepare antibiotic stock solutions.", "checked": false, "checklist_id": 963, "created_at": "2018-12-21T11:12:20.831Z", "updated_at": "2018-12-21T11:12:20.831Z", "created_by_id": null, "last_modified_by_id": null, "position": 1 }, { "id": 3964, "text": "Streak the bacterial isolates to be tested onto nutrient-rich agar plates without inhibitor to obtain single colonies", "checked": false, "checklist_id": 963, "created_at": "2018-12-21T11:12:20.838Z", "updated_at": "2018-12-21T11:12:20.838Z", "created_by_id": null, "last_modified_by_id": null, "position": 2 } ] } ], "step_comments": [], "step_assets": [ { "id": 3087, "step_id": 4363, "asset_id": 3749 } ], "assets": [ { "asset": { "id": 3749, "created_at": "2019-02-20T07:37:48.184Z", "updated_at": "2019-02-20T07:38:26.671Z", "created_by_id": 202, "last_modified_by_id": null, "estimated_size": 48768, "lock": null, "lock_ttl": null, "version": 1, "file_processing": false, "team_id": 1 }, "asset_blob": { "filename": "Plate_Streaking.jpg" } } ], "step_tables": [], "tables": [] }, { "step": { "id": 4364, "name": "Incubate the broth", "description": "\n\n

Incubation conditions:

\n\n
Culture growth will require 2–6 h depending on the growth rate of the bacteria to be tested. This pause period can be used to prepare the antibiotic or peptide dilutions.", "position": 2, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T10:35:37.405Z", "updated_at": "2019-02-19T14:43:59.634Z", "last_modified_by_id": 202, "protocol_id": 3452 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] }, { "step": { "id": 4361, "name": "Assessment of turbidity", "description": "\n\nTurbidity can be assessed visually by comparing the test and the McFarland Standard.

Mix the McFarland 0.5 BaSO4 standard vigorously using a vortex mixer. The turbidity is verified measuring the absorbance of the suspension spectrophotometrically. The absorbance should be in the same range as that of the McFarland standard 0.5 (OD625 nm should be at 0.08–0.13).", "position": 3, "completed": false, "completed_on": null, "user_id": 202, "created_at": "2018-11-09T10:25:56.657Z", "updated_at": "2019-02-19T14:44:41.586Z", "last_modified_by_id": 202, "protocol_id": 3452 }, "checklists": [], "step_comments": [], "step_assets": [], "assets": [], "step_tables": [], "tables": [] } ] } ], "results": [] } ], "my_module_groups": [ { "id": 1185, "created_at": "2018-12-19T13:28:00.792Z", "updated_at": "2018-12-19T13:28:00.792Z", "created_by_id": 3, "experiment_id": 430 } ] }