scinote-web/app/assets/templates/experiment_433/experiment.json
2019-02-21 15:06:34 +01:00

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{"experiment":{"id":433,"name":"Development of SNP markers panel","description":"This template shows how to generate a SNP markers panel with the preparation of SNP panel.\r\nSingle nucleotide polymorphisms (SNPs) markers are widely used for genomic research and breeding. 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sequencing.","checked":false,"checklist_id":960,"created_at":"2018-12-21T10:22:55.989Z","updated_at":"2018-12-21T10:23:18.774Z","created_by_id":null,"last_modified_by_id":null,"position":3}]}],"step_comments":[],"step_assets":[{"id":3084,"step_id":4422,"asset_id":3746},{"id":3085,"step_id":4422,"asset_id":3747}],"assets":[{"id":3746,"created_at":"2019-02-20T07:36:46.773Z","updated_at":"2019-02-20T07:37:25.383Z","file_file_name":"Shearing.jpg","file_content_type":"image/jpeg","file_file_size":17969,"file_updated_at":"2019-02-20T07:37:24.952Z","created_by_id":202,"last_modified_by_id":null,"estimated_size":19765,"file_present":true,"lock":null,"lock_ttl":null,"version":1,"file_processing":false,"team_id":1},{"id":3747,"created_at":"2019-02-20T07:36:46.946Z","updated_at":"2019-02-20T07:37:25.757Z","file_file_name":"Amplification.jpg","file_content_type":"image/jpeg","file_file_size":15037,"file_updated_at":"2019-02-20T07:37:25.431Z","created_by_id":202,"last_modified_by_id":null,"estimated_size":16540,"file_present":true,"lock":null,"lock_ttl":null,"version":1,"file_processing":false,"team_id":1}],"step_tables":[],"tables":[]},{"step":{"id":4420,"name":"RAD 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volume\u003c/li\u003e\n\u003cli\u003eThe samples are heat-inactivated at \u003cstrong\u003e65°C\u003c/strong\u003e for \u003cstrong\u003e20 min\u003c/strong\u003e.\u003c/li\u003e\n\u003c/ul\u003e\n\u003cbr\u003e\u003cstrong\u003eIndividual-specific P1 adapters, each with a unique 5 or 7 bp barcode is ligated to the digested DNA at 22°C for 15 min by adding:\u003c/strong\u003e\u003cbr\u003e\u003cbr\u003e\n\u003cul\u003e\n\u003cli\u003e0.6 μL (DNA samples) 100 nmol/L P1 adapter\u003c/li\u003e\n\u003cli\u003e0.15 μL 100 mmol/L rATP \u003c/li\u003e\n\u003cli\u003e0.25 μL 10× Reaction Buffer 2\u003c/li\u003e\n\u003cli\u003e0.125 μL T4 ligase \u003c/li\u003e\n\u003cli\u003ereaction volumes made up to 15 μL with nuclease-free water \u003c/li\u003e\n\u003c/ul\u003e\n\u003cbr\u003eAfter heat-inactivation, the ligation reactions are slowly cooled to room temperature then combined in appropriate multiplex 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