scinote-web/app/assets/templates/experiment_430/experiment.json
2019-11-18 14:26:38 +01:00

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{
"experiment": {
"id": 430,
"name": "Determination of (MIC) by agar and broth dilution",
"description": "With this template, minimum inhibitory concentrations (MICs) can be determined. MICs are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation. \r\nThe aim of broth and agar dilution methods is to determine the lowest concentration of the assayed antimicrobial agent (minimal inhibitory concentration, MIC) that, under defined test conditions, inhibits the visible growth of the bacterium being investigated.MIC values are used to determine the susceptibilities of bacteria to drugs and also to evaluate the activity of new antimicrobial agents. Agar dilution involves the incorporation of different concentrations of the antimicrobial substance into a nutrient agar medium followed by the application of a standardized number of cells to the surface of the agar plate. \r\nDilution methods are used to MICs of antimicrobial agents and are the reference methods for antimicrobial susceptibility testing.",
"project_id": 223,
"created_by_id": 202,
"last_modified_by_id": 202,
"archived": false,
"archived_by_id": null,
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"created_at": "2018-11-09T08:38:03.789Z",
"updated_at": "2019-02-07T14:55:25.517Z",
"uuid": "f2949887-1f9e-4eb8-b645-ebbdd8808caf"
},
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},
"protocol_protocol_keywords": [],
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{
"step": {
"id": 4388,
"name": "Troubleshooting table",
"description": "<!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\">\n<html><body><p>Look at the table for troubleshooting.</p></body></html>",
"position": 0,
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"created_at": "2018-11-09T14:44:17.342Z",
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}
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}
]
}
]
}
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"name": "Cell count",
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"id": 4375,
"name": "Sampling",
"description": "<!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\">\n<html><body><p>To confirm that the size of the bacterial inoculum was appropriate, determine the viable count of the bacterial suspension used for preparing the initial inoculum. <br><br><br></p></body></html>",
"position": 0,
"completed": false,
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"created_at": "2018-11-09T13:29:23.180Z",
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"checked": false,
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"created_at": "2018-12-21T12:05:41.325Z",
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{
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"text": "Dilute this solution sequentially 1:10 three times until you reach a dilution of 105.",
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},
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"checked": false,
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"created_at": "2018-12-21T12:05:41.345Z",
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"step": {
"id": 4377,
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"description": "<!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\">\n<html><body><p>Count colonies on plates (taking into account only plates with up to <strong>500</strong> colonies).<br>It is best to determine the relationship between <strong>OD600</strong> and the microbial number basing the calculation on plates displaying a number of colonies between <strong>100</strong> and <strong>400</strong>. <strong>Higher numbers do not accurately represent the original suspension as errors created by coincidence of colonies and nutrient limitation lead to numbers that are lower than expected.</strong> Relying on fewer than <strong>100</strong> colonies might, on the other hand, lead to incorrect conclusions due to the addition of statistical and methodological errors while performing the dilutions.</p></body></html>",
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"description": "<!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\">\n<html><body>\n<p><strong>Incubation parameters:</strong></p>\n<ul>\n<li>37°C</li>\n<li>16-20h</li>\n</ul>\n</body></html>",
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"description": "<!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\">\n<html><body><p>Calculate the CFU per mL that were in the overnight culture according to the following formula, where <strong>N=(Cx10)/10<sup>-D</sup> (CFU/mL)</strong>; C= number of colonies per plate; D= number of 1:10 dilution.<br><br>Average the results from three tests performed with the microbial isolate and correlate this number with the OD600 value obtained from the overnight culture. This relationship holds true for subsequent cultures of the same bacterium grown in the same way.</p></body></html>",
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{
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"name": "MIC agar dilutions",
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"description": "<!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\">\n<html><body><p>In order for the test to be valid, the agar plates for the cell count have to be checked to verify that the right number of CFU were used. The presence of around <strong>100200 colonies</strong> on the lower of the two dilutions<strong> (10<sup>-5</sup>)</strong> of the initial bacterial suspension is expected when using the correct bacterial suspension density of <strong>12x10<sup>8</sup> CFU/mL</strong>. If the cell numbers are within the desired range, the test can be analyzed to determine the MIC. Also, check the antibiotic-free growth control plate. Visible growth needs to occur for the test to be valid.<br><br><em><strong>The MIC is defined as the lowest concentration of the antimicrobial substance that inhibits visible growth of the tested isolate.</strong></em> The growth of a single colony or faint film caused by the inoculum should be disregarded. When growth of the tested organism occurs on all agar plates with antimicrobial agent, the MIC is recorded as greater than the highest concentration tested. The MIC is recorded as less than or equal to the lowest concentration when no growth occurs on any of the agar plates but the growth control.</p></body></html>",
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},
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"asset_blob": {
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