{"experiment":{"id":440,"name":"Microbiological sampling_QA","description":"Quality assurance (QA) is a process, where primarily concerns are controls of errors in the performance of tests and verification of test results. All materials, equipment and procedures must be adequately controlled. \r\nThe objectives of microbiological sampling are to allow statements of density, types and locations of microorganism which reside on different surfaces.","project_id":223,"created_by_id":202,"last_modified_by_id":202,"archived":false,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2018-11-15T09:56:28.245Z","updated_at":"2019-01-31T09:56:15.702Z","workflowimg_file_name":"wimg20190131-1-rg9zf4.png","workflowimg_content_type":"image/png","workflowimg_file_size":5190,"workflowimg_updated_at":"2019-01-31T09:56:15.702Z","uuid":"eef319cd-8f18-4ec8-809b-ca44336c85b5"},"my_modules":[{"my_module":{"id":2548,"name":"Gram Stain Test","due_date":null,"description":null,"x":0,"y":50,"my_module_group_id":null,"created_at":"2019-01-31T09:55:55.557Z","updated_at":"2019-01-31T09:55:55.557Z","archived":false,"archived_on":null,"created_by_id":202,"last_modified_by_id":202,"archived_by_id":null,"restored_by_id":null,"restored_on":null,"nr_of_assigned_samples":0,"workflow_order":-1,"experiment_id":440,"state":"uncompleted","completed_on":null,"electronic_signature_status":"unlocked","electronic_signature_status_locked_at":null},"outputs":[],"my_module_tags":[],"task_comments":[],"my_module_repository_rows":[],"user_my_modules":[],"protocols":[{"protocol":{"id":4193,"name":null,"authors":null,"description":null,"added_by_id":null,"my_module_id":2548,"team_id":1,"protocol_type":"unlinked","parent_id":null,"parent_updated_at":null,"archived_by_id":null,"archived_on":null,"restored_by_id":null,"restored_on":null,"created_at":"2019-01-31T09:55:55.566Z","updated_at":"2019-02-01T14:19:25.278Z","published_on":null,"nr_of_linked_children":0},"protocol_protocol_keywords":[],"steps":[{"step":{"id":5475,"name":"Reagents","description":"\u003c!DOCTYPE html PUBLIC \"-//W3C//DTD HTML 4.0 Transitional//EN\" \"http://www.w3.org/TR/REC-html40/loose.dtd\"\u003e\n\u003chtml\u003e\u003cbody\u003e\n\u003cp\u003eReagents bellow can be made or purchased.\u003cbr\u003e\u003cstrong\u003e\u003cbr\u003eCrystal Violet Staining Reagent:\u003cbr\u003e\u003c/strong\u003e\u003cspan style=\"text-decoration: underline;\"\u003e\u003cbr\u003eSolution A for crystal violet staining reagent\u003c/span\u003e\u003cbr\u003e\n\u003c/p\u003e\n\u003cul\u003e\n\u003cli\u003eCrystal violet (certified 90% dye content), 2g\u003c/li\u003e\n\u003cli\u003eEthanol, 95% (vol/vol), 20 mL\u003c/li\u003e\n\u003c/ul\u003e\n\u003cspan style=\"text-decoration: underline;\"\u003eSolution B for crystal violet staining reagent\u003cbr\u003e\u003c/span\u003e\n\u003cul\u003e\n\u003cli\u003eAmmonium oxalate, 0.8 g\u003c/li\u003e\n\u003cli\u003eDistilled water, 80 mL\u003c/li\u003e\n\u003c/ul\u003e\nMix A and B to obtaincrystal violet staining reagent.Store for 24 h and filter through paper prior to use.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eGram's Iodine:\u003c/strong\u003e\u003cbr\u003e\n\u003cul\u003e\n\u003cli\u003eIodine, 1.0 g\u003c/li\u003e\n\u003cli\u003ePotassium iodide, 2.0 g\u003c/li\u003e\n\u003cli\u003eDistilled water, 300 mL\u003c/li\u003e\n\u003c/ul\u003e\nGrind the iodine and potassium iodide in a mortar and add water slowly with continuous grinding until the iodine is dissolved. Store in amber bottles.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSafranin:\u003cbr\u003e\u003cbr\u003e\u003c/strong\u003e\u003cspan style=\"text-decoration-line: underline;\"\u003eStock solution:\u003c/span\u003e\u003cbr\u003e\n\u003cul\u003e\n\u003cli\u003e2.5g Safranin O\u003c/li\u003e\n\u003cli\u003e100 ml 95% Ethanol\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eWorkingSolution:\u003c/span\u003e\u003c/p\u003e\n\u003cul\u003e\n\u003cli\u003e10mlStockSolution\u003c/li\u003e\n\u003cli\u003e90mlDist
